Tuesday, May 22, 2012

Cryopreservation of banana

One of the practical solution for long term conservation of banana germplasm is cryopreservation or freeze preservation of meristems culture in liquid nitrogen.

It is a promising option for safe, long-term storage of Musa germplasm.

The method involves 2-4 weeks preculture of proliferating meristems on a high sucrose medium. Surviving peristematic clumps are then excised transferred to cryotubes, and plunged into liquid nitrogen for rapid freezing (-196 ° C).

At this temperature, cell division and metabolic process stop and the plant material can therefore be stored without modification for a long period of time.

An ideal embryogenic cell suspension contains a high proportion of ells that are isodiametric and characterized by a relatively large nucleus, small multiple vacuoles and tiny starch and protein grains.

Cryopreservation is the most effective means available to secure the integrity of the collection over long term.
Cryopreservation of banana

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